CD31 Rabbit Monoclonal Antibody(C1959)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
IHC | 1:100 - 1:300 |
IF/ICC | 1:100 - 1:300 |
IP | 1:50 - 1:100 |
Description | Recombinant rabbit monoclonal antibody to CD31 |
Specificity | Recognizes endogenous levels of CD31 protein |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | Recombinant fusion protein of human CD31. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 82 kD; Observed: 130 kD |
Form/Buffer | Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300. |
Alternative Names | Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA; PECA1; CD31 |
Gene Symbol | PECAM1 |
Entrez Gene | 5175(Human); 18613(Mouse); 29583(Rat) |
SwissProt | P16284(Human); Q08481(Mouse); Q3SWT0(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of CD31 expression in HepG2 (A), mouse brain (B), rat spleen (C) whole cell lysates. (Predicted band size: 82 kD; Observed band size: 130 kD)
Immunohistochemical analysis of CD31 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with TE buffer (pH 9.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CD31 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of CD31 staining in mouse skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with TE buffer (pH 9.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AREX® Fluor 488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
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