CD31 Rabbit Monoclonal Antibody(ARA812)

Datasheet

MSDS

Key features and details

Target: CD31
Source/Host: Rabbit
Reactivity: Human
Clonality: Monoclonal
Applications: WB,IHC,FC,IP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6599

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Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions under most inflammatory conditions . Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes . Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions . Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils . Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:1000-1:2000
IHC	1:1600-1:3200
FC	1:200-1:1000
IP	1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to CD31
Antibody Type	Primary antibody
Predicted MW	83kDa
Immunogen	A synthetic peptide corresponding to residues on the C-terminus of human CD31 was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.87%.
Alternative Names	Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD31
Gene Symbol	PECAM1
Entrez Gene	5175(Human)
Swissprot	P16284

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-CD31 antibody at 1:1,000 dilution
Predicted MW: 83 kDa
Observed MW: 130 kDa
Lane 1: A431
Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 60s

All lanes: Anti-CD31 antibody at 1:1,000 dilution
Predicted MW: 83 kDa
Observed MW: 130 kDa
Lane 1: THP-1
Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 20s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling CD31 with ARA812 at 1:3,200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Overlay histogram showing Jurkat cells stained with ARA812 (Red). The cells were fixed with 4% paraformaldehyde for 10 min. The cells were then incubated in the antibody (ARA812, 1:1,000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor<sup>®</sup> 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

CD31 was immunoprecipitated from 0.4mg of THP-1 whole cell lysate with ARA812 at 1:50 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: ARA812 IP in THP-1 whole cell lysate
Lane 2: PBS instead of ARA812 in THP-1 whole cell lysate
Lane 3: THP-1 whole cell lysate, 10 µg (input)
Exposure: 120s

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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