CD299 Mouse Monoclonal Antibody(C2643)

MSDS

Key features and details

Mouse monoclonal to CD299

Target: CD299
Source/Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Applications: WB, FC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA02255

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
FC	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Mouse monoclonal to CD299
Specificity	Recognizes endogenous levels of CD299 protein
Antibody Type	Primary antibody
Imnunogen	Recombinant fusion protein of human CD299 expressed in E. Coli
Purification	This antibody is purified through a protein G column.
Molecular Weight	Predicted: 45 kD; Observed: 55 kD kD
Form/Buffer	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	CD209L; CD209L1; CD299; C-type lectin domain family 4 member M; CD209 antigen-like protein 1; DC-SIGN-related protein; DC-SIGNR; DendritIC, cell-specifIC, IC,AM-3-grabbing non-integrin 2; DC-SIGN2; Liver/lymph node-specifIC, IC,AM-3-grabbing non-integrin; L-SIGN; CD299
Gene Symbol	CLEC4M
Entrez Gene	10332(Human)
SwissProt	Q9H2X3(Human)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of CD299 expression in L02 (A), HepG2 (B), BEL7402 (C), SMMC7702 (D), HL7702 (E) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 55 kD kD)

Flow cytometric analysis of MOLT4 cells using Anti-CD299 Antibody (green) and negative control (red).

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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