CD257 Mouse Monoclonal Antibody(C3003)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:250 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to CD257 |
Specificity | Recognizes endogenous levels of CD257 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human CD257 expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 31 kD; Observed: 31 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | BAFF; BLYS; TALL1; TNFSF20; ZTNF4; Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; DendritIC, cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1; CD257 |
Gene Symbol | TNFSF13B |
Entrez Gene | 10673(Human) |
SwissProt | Q9Y275(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of CD257 expression in SKNSH (A), MOLT4 (B) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 31 kD kD)
Immunofluorescent analysis of CD257 staining in SMMC7721 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HeLa cells using Anti-CD257 Antibody (green) and negative control (red).
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