CD22 Rabbit Monoclonal Antibody(C671)
MSDS 
Key features and details
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Product Details
IHC | 1:100 - 1:500 |
IF/ICC | 1:50 - 1:200 |
Description | Recombinant rabbit monoclonal antibody to CD22 |
Specificity | Recognizes endogenous levels of CD22 protein |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | Recombinant fusion protein of human CD22. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | N/A |
Form/Buffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.05% BSA and 0.01% sodium azide. |
Alternative Names | SIGLEC2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD22 |
Gene Symbol | CD22 |
Entrez Gene | 933(Human) |
SwissProt | P20273(Human) |
Immunohistochemical analysis of CD22 staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CD22 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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