CD19 Rabbit Monoclonal Antibody(ARA784)

Datasheet

MSDS

Key features and details

Target: CD19
Source/Host: Rabbit
Reactivity: Human
Clonality: Monoclonal
Applications: WB,IHC,IP,FC,IF/ICC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6571

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes on B-lymphocytes . Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens . Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores . Is not required for early steps during B cell differentiation in the blood marrow . Required for normal differentiation of B-1 cells . Required for normal B cell differentiation and proliferation in response to antigen challenges . Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:2000-1:10000
IHC	1:200-1:400
IF/ICC	1:10-1:50
FC	1:50-1:100
IP	1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to CD19
Antibody Type	Primary antibody
Predicted MW	61kDa
Immunogen	A synthetic peptide corresponding to the C-term of CD19 was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.59%.
Alternative Names	B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19
Gene Symbol	CD19
Entrez Gene	930(Human)
Swissprot	P15391

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-CD19 antibody at 1:2,000 dilution
Predicted MW: 61 kDa
Observed MW: 95 kDa

Lane 1: Ramos
Lane 2: Daudi
Lane 3: Raji

Lysate at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 10s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of spleen tissue labelling CD19 with ARA784 at 1:400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Anti-CD19 was immunoprecipitated from 0.4mg of Ramos lysate with ARA784 at 1:50 dilution.
2nd Ab:
GAR HRP for IP 1:1,000

Lane 1: ARA784 IP in Ramos whole cell lysate
Lane 2: Rabbit IgG instead of ARA784 in Ramos whole cell lysate
Lane3: Ramos whole cell lysate, 10 µg(input)

Exposure: 60s

Overlay histogram showing Ramos cells stained with ARA784 (Blue). The cells were fixed with 4% paraformaldehyde for 10 mins. The cells were then incubated in the antibody (ARA784, 1:10 dilution) in 1x PBS/1% BSA for 30 min at room temperature . The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Red) was used as a control.

ARA784 staining CD19 in Raji cells by IF/ICC (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:50) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.

Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(1:500).

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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