CD138 Mouse Monoclonal Antibody(C3715)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:500 - 1:1000 |
Description | Mouse monoclonal antibody to CD138 |
Specificity | Recognizes endogenous levels of CD138 protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide of human CD138. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 32 kD; Observed: 70 kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | SDC; Syndecan-1; SYND1; CD138 |
Gene Symbol | SDC1 |
Entrez Gene | 6382(Human) |
SwissProt | P18827(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of CD138 expression in Hela (A), HepG2 (B) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 70 kD)
Immunofluorescent analysis of CD138 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HepG2 cells using Anti-CD138 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
FAQs
New Products
