CD10 Rabbit Monoclonal Antibody(ARB836)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARB6626
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Size:
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Product Details
Background
CD10 is a single-chain cell surface glycoprotein, also is designated as common acute lymphoblastic leukaemia antigen (CALLA), neprilysin and neutral endopeptidase. Besides, CD10 is a zink-dependent peptidase (metalloprotease), degrading various bioactive peptides, and it plays a functional role by modulating cellular responses to peptide substrates.CD10 is present on the cell surface of bone marrow stem cells and myelopoietic cells (including neutrophils), follicular centre cells, few mature B-lymphocytes, and a subpopulation of parafollicular T-lymphocytes. CD10 is also found in enterocytes in the upper part of the intestinal tract (brush border), in liver (bile canaliculi), kidney (glomerular and proximal tubular cells), pulmonary alveolar cells, myoepithelial cells of breast and sweat and salivary glands, prostate glandular cells, placental rophoblastic cells, endometrial stromal cells, some endothelial cells, and a minority of (myo-)fibroblasts (including skin periadnexal cells). CD10 is expressed in a high percentage of cases of acute lymphoblastic leukemia, follicular lymphoma, Burkitt's lymphoma, some hematopoietic tumors, and chronic myelogenous leukemias in lymphoid blast crisis.CD10 is particularly useful in the classification of B-cell leukaemias/lymphomas and classification of carcinomas (identification of hepatocellular carcinoma and renal cell carcinoma). CD10 may be used in the identification of metaplastic breast carcinoma, prognostication of breast carcinoma, and classification of uterine mesenchymal neoplasms (identification of stromal sarcoma).
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Predicted Molecular Wt | 86 kDa |
Purity | ProA affinity purified IgG |
Subcellular location | Membrane |
Swissprot ID | P08473 |
Immunogen | Recombinant protein corresponding to CD10 residues within aa1-500 |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Recommended method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18° C-25° C) for 30 minutes. |
Alternative Names | EPN; Neprilysin; Atriopeptidase; Common acute lymphocytIC, leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD10 |
Gene Symbol | MME |
Entrez Gene | 4311 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human tonsil tissue sections was performed using CD10 Rabbit Monoclonal Antibody (ARB836).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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