CD10 Rabbit Monoclonal Antibody(ARA790)

Datasheet

MSDS

Key features and details

Target: CD10
Source/Host: Rabbit
Reactivity: Human, Rat
Clonality: Monoclonal
Applications: WB,IHC,FC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6577

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Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids . Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond . Catalyzes cleavage of bradykinin, substance P and neurotensin peptides . Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 . Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) . Displays UV-inducible elastase activity toward skin preelastic and elastic fibers .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:2000-1:5000
IHC	1:3200-1:6400
FC	1:800-1:2000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to CD10
Antibody Type	Primary antibody
Predicted MW	86kDa
Immunogen	A recombinant protein corresponding to CD10 residues within aa1-500 was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.65%.
Alternative Names	EPN; Neprilysin; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD10
Gene Symbol	MME
Entrez Gene	4311(Human); 17380(Mouse); 24590(Rat)
Swissprot	P08473

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-CD10 antibody at 1:2,000 dilution
Predicted MW: 86 kDa
Observed MW: 100 kDa

Lane 1: LnCap
Lane 2: Rat Kidney

Lysate at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 60s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of appendix tissue labelling CD10 with ARA790 at 1:6,400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Overlay histogram showing Raji cells stained with ARA790 (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA790, 1:2,000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor<sup>®</sup> 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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