C9RANT Mouse Monoclonal Antibody(ARA714)

Datasheet

MSDS

C9RANT Mouse Monoclonal Antibody(ARA714)

Key features and details

Target: C9RANT
Host: Mouse
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: WB, IHC, ICC/IF
Storage: -20°C

Brand:

CAT.NO. : ARA6482

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:500 - 1:1000
IHC	1:500 - 1:2000
IF/ICC	1:200 - 1:800

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Species Reactivity	Human, Mouse, Rat
Immunogen	Recombinant human C9orf72 protein
Isotype	Mouse / IgG2a
Antibody Class	Monoclonal
Alternative Names	Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT
Calculated Molecular Weight	481 aa, 54 kDa
Observed Molecular Weight	55 kDa
Gene Name	C9orf72
Gene ID (NCBI)	203228
Conjugation	Unconjugated
Form	Liquid

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Neuro-2a cells were subjected to SDS PAGE followed by western blot with C9RANT Mouse Monoclonal Antibody(ARA714) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human brain using C9RANT Mouse Monoclonal Antibody(ARA714) at dilution of 1:50 (under 10x lens).

Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using C9RANT Mouse Monoclonal Antibody(ARA714) at dilution of 1:400 and Flour 488-Conjugated Goat Anti-Mouse IgG(H+L) .

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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