BNIP2 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:100 - 1:500 |
FC | 1:100 - 1:200 |
Description | Rabbit polyclonal antibody to BNIP2 |
Specificity | Recognizes endogenous levels of BNIP2 protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BNIP2. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 36 kD; Observed: 36 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | NIP2; BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 |
Gene Symbol | BNIP2 |
Entrez Gene | 663(Human); 12175(Mouse) |
SwissProt | Q12982(Human); O54940(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of BNIP2 expression in mouse testis (A), rat testis (B) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 36 kD)
Immunofluorescent analysis of BNIP2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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