Beta III Tubulin Mouse Monoclonal Antibody(ARA835)

Datasheet

MSDS

Beta III Tubulin Mouse Monoclonal Antibody(ARA835)

Key features and details

Target: Beta III Tubulin
Host: Mouse
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: WB, IF, IHC-P, FC
Storage: -20°C

Brand:

CAT.NO. : ARA6649

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Tubulin is a compound of subunits of A tubulin and B tubulin. Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:2,000-1:5,000
IF-Cell	1:500-1:1,000
IHC	1:1,500-1:2,000
FC	1:800-1:1,000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Product Name	Beta III Tubulin Mouse Monoclonal Antibody (ARA835)
Antibody Type	Mouse Monoclonal Antibody
Immunogen	Synthetic peptide (KLH-coupled) within human Tubulin beta-3 chain aa 401-450.
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IF-Cell, IHC-P, FC, IF-Tissue
Molecular Weight	Predicted band size: 50 kDa
Positive Control	SH-SY5Y cell lysate, U-87 MG cell lysate, A-172 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HEK-293, SH-SY5Y, PC-12, Neuro-2a, human brain tissue, mouse brain tissue, rat brain tissue, mouse hippocampus tissue, MCF7.
Conjugation	unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Beta III Tubulin on different lysates with Mouse anti-Beta III Tubulin antibody (ARA835) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-Beta III Tubulin antibody (ARA835) at 1/2,000 dilution.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Beta III Tubulin antibody (ARA835) at 1/2,000 dilution.

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Beta III Tubulin antibody (ARA835) at 1/2,000 dilution.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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