Beta galactosidase Rabbit Monoclonal Antibody(ARA681)
Key features and details
- Reactivity:
- Application:
- Host:
- Clonality:
- Target:
-
Brand:
CAT.NO. : ARA6395
US$ Please choose
US$ Please choose
Product Details
Background
GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). The MW of GLB1 after glycosylation is 100-120 kd. GLB1 is prone to produce homodimers (220-240 kd) and higher multimers (PMID: 3926488). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda, and 61 kDa.
Application
|
Application |
Dilution Ratio |
|
WB |
1:2000-1:50000 |
|
IF/ICC |
1:100-1:500 |
|
FC |
0.25 ug per 10^6 cells in a 100 µl suspension |
Overview
|
Product Description |
Recombinant Rabbit monoclonal antibody to Beta-galactosidase |
|
Immunogen |
Recombinant human Beta galactosidase protein |
|
Purification Method |
Protein A purification |
|
Clonality |
Monoclonal |
|
Form |
Liquid |
|
Storage Buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
|
Validated Applications |
WB, IF/ICC, FC, ELISA |
|
Reactivity Species |
Human |
|
Host/Isotype |
Rabbit / IgG |
|
Conjugation |
Unconjugated |
|
Calculated Molecular Weight |
76 kDa |
|
Observed Molecular Weight |
62-67 kDa |
|
Gene Symbol |
GLB1/Beta-galactosidase |
|
Gene ID (NCBI) |
2720 |
|
Alternative Names |
GLB1, 240788F1, Acid beta-galactosidase, Beta-galactosidase, EBP |
|
UNIPROT ID |
P16278 |
Data
WB result of Beta galactosidase Rabbit Monoclonal Antibody(ARA681); incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta galactosidase transfected A549 cells.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta galactosidase Rabbit Monoclonal Antibody(ARA681) and AREX®Flour 488 Rabbit IgG(H+L) Goat Antibody.
1x10^6 A549 cells were intracellularly stained with 0.25 ug Beta galactosidase Rabbit Monoclonal Antibody(ARA681) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
New Products
