Beta Actin Rabbit Monoclonal antibody(ARA710)

Datasheet

MSDS

Beta Actin Rabbit Monoclonal antibody(ARA710)

Key features and details

Target: Beta Actin
Host: Rabbit
Reactivity: Human, Mouse, Rat, Pig
Clonality: Monoclonal
Application: WB, IHC, IF/ICC, IP
Storage: -20°C

Brand:

CAT.NO. : ARA6450

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:5000 - 1:40000
IHC	1:1000 - 1:5000
IF/ICC	1:500 - 1:4000
IP	0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Reactivity	Human, Mouse, Rat, Pig
Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Recombinant human beta actin protein
Full Name	actin, beta
Calculated Molecular Weight	375 aa, 42 kDa
Observed Molecular Weight	42 kDa
Gene Symbol	Beta Actin
Gene ID (NCBI)	60
Conjugate	Unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Various lysates were subjected to SDS PAGE followed by western blot with Beta Actin Rabbit Monoclonal antibody(ARA710) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using Beta Actin Rabbit Monoclonal antibody(ARA710) at dilution of 1:2000 . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Beta Actin Rabbit Monoclonal antibody(ARA710) at dilution of 1:1900 and Fluor 488-Conjugated AffiniPure Rabbit IgG(H+L) Goat Antibody.

IP result of Beta Actin Rabbit Monoclonal antibody(ARA710) (IP:4ug; Detection:1:5000) with HeLa cells lysate 1400 ug.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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