BAX Rabbit Polyclonal Antibody
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
-
Brand:
CAT.NO. : ARA6372
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Product Details
Background
BAX (also known as BCL2 Associated X, Bcl-2-Like Protein 4, Bcl2-L-4, BCL2L4) is a member of the BCL2 family of proteins that play a key role in the regulation of apoptosis in higher eukaryotes. BAX comprises 4 Bcl-2 homology domains (BH1-BH4) and a C-terminal transmembrane domain. In healthy mammalian cells, BAX is localized to the cytoplasm through its interaction with the anti-apoptotic BL-2 family members BCL2L1/Bcl-xL (PMIDs: 28755482, 21458670). In response to apoptotic stimuli, however, BAX undergoes a conformational change that causes it to translocate to the outer mitochondrial membrane where it initiates the mitochondrial pathway of apoptosis via two potential mechanisms. Firstly, upon translocation to the outer mitochondrial membrane, BAX interacts with the mitochondrial voltage-dependent anion channel (VDAC) leading to the opening of the channel, loss of membrane potential, and the release of cytochrome c from the mitochondrion (PMID:10766872). The release of cytochrome C into the cytoplasm leads to the activation of Caspase3, initiating apoptosis. Secondly, activated BAX forms homodimers, which then assemble into oligomers on the mitochondrial outer membrane to create pores that permeabilize the mitochondrion leading to the release of cytochrome C.
Application
|
Application |
Dilution Ratio |
|
WB |
1:20000 - 1:100000 |
|
IHC |
1:1000 - 1:4000 |
|
IF/ICC |
1:50 - 1:500 |
|
IP |
0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Overview
|
Product Description |
Rabbit Polyclonal Antibody to BAX |
|
Immunogen |
Recombinant human BAX protein |
|
Purification Method |
Protein A purification |
|
Clonality |
Polyclonal |
|
Form |
Liquid |
|
Storage Buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
|
Host/Isotype |
Rabbit / IgG |
|
Validated Applications |
WB, IHC, IP, IF/ICC |
|
Reactivity Species |
Human, Mouse, Rat, Rabbit, Monkey, Chicken, Zebrafish, Hamster, Sheep, Goat |
|
Molecular Weight |
21 kDa |
|
Gene Symbol |
BAX |
|
Alternative Names |
Apoptosis regulator BAX, Bcl2-L-4, Bcl-2-like protein 4 |
|
Conjugation |
Unconjugated |
|
UNIPROT ID |
Q07812 |
Data
Various lysates were subjected to SDS PAGE followed by western blot with BAX Rabbit Polyclonal Antibody incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using BAX Rabbit Polyclonal Antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BAX Rabbit Polyclonal Antibody at dilution of 1:200 and AREX®Flour 488 Rabbit IgG(H+L) Goat Antibody.
IP result of BAX Rabbit Polyclonal Antibody with mouse heart tissue lysate 1680 ug.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
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