ATP1A1/ATP1A2/ATP1A3 Mouse Monoclonal Antibody (H-3)

Datasheet

MSDS

ATP1A1/ATP1A2/ATP1A3 Mouse Monoclonal Antibody (H-3)

Key features and details

Target: ATP1A1/ATP1A2/ATP1A3
Host: Mouse
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: WB, IP, IF, IHC(P) , ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA1233

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

The ubiquitously expressed sodium/potassium - ATPase (Na⁺/K⁺ - ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na⁺ ions and two K⁺ ions against their respective electrochemical gradients. As a member of the P - type family of ion motives, Na⁺/K⁺ - ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na⁺ - coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na⁺/K⁺ - ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co - localizes with the α subunit in nephron segments, where it increases the affinity of Na⁺/K⁺ - ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na⁺/K⁺ - ATPase.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:2000-1:10000
IHC	1:50-1:500
IF/ICC	1:50-1:500
ELISA	1:30-1:3000
IP	1-2 µg/100-500 µg total protein

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Isotype	Mouse IgG2b kappa light chain
Target Antigen	Na+/K+ ATPase alpha (recognizes alpha1, alpha2, and alpha3 isoforms)
Gene Name	ATP1A1, ATP1A2, ATP1A3
UniProt ID	P05023 (Human ATP1A1), P50993 (Human ATP1A2), P13637 (Human ATP1A3)
Entrez Gene ID	476 (Human ATP1A1), 477 (Human ATP1A2), 478 (Human ATP1A3)
Molecular Weight	100-113 kDa
Gene Aliases	Sodium/potassium-transporting ATPase subunit alpha
Immunogen	amino acids 551-850 of Na+/K+ ATPase alpha1 of human origin
Form/Buffer	PBS with < 0.1% sodium azide and 0.1% gelatin

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Direct fluorescent western blot analysis of Na⁺/K⁺ - ATPase α expression in PC - 12 (A), Hep G2 (B) and MDCK (C) whole cell lysates and human kidney (D) and human brain (E) tissue extracts.

Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

Immunofluorescence staining of methanol - fixed HeLa cells showing membrane localization (above). Immunoperoxidase staining of formalin fixed, paraffin - embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli in membrane and cytoplasmic staining of cells in tubules (below).

Storage

Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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