Anti-Infectious Bronchitis Virus (IBV) S Protein Mouse Monoclonal Antibody (IBV-S_6H8)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : AXA0020
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Size:
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Product Details
Background
The Infectious Bronchitis Virus (IBV) S Protein, also known as Spike Protein, is a key glycoprotein located on the surface of the virus that plays a crucial role in the viral life cycle primarily responsible for recognizing and binding to specific receptors on host cells thereby mediating viral entry into the host cells it consists of two subunits, S1 and S2 where the S1 subunit determines the viral tissue tropism and serotype specificity due to its high variability which is the main reason for the differentiation of different IBV serotypes meanwhile as the major antigen inducing the host to produce neutralizing antibodies the IBV S Protein is also a core target for the development of vaccines and diagnostic reagents.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:200-1:1000 |
IF/ICC | 1:200-1:400 |
Overview
Description | Mouse Monoclonal antibody to Infectious Bronchitis Virus (IBV) S Protein |
Antibody Type | Primary antibody |
Immunogen | Prokaryotically expressed S Protein of IBV QX Strain |
Form/Buffer | PBS, 20% Glycerol; preservative: 0.05% Sodium Azide |
Alternative Names | IBV S, IBV Spike, Infectious bronchitis virus spike glycoprotein, IBV S1/S2 protein |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
The WB results showed that this antibody can effectively recognize the S protein expressed in sf9 cells infected with baculovirus pfast-IBV S and the CEK cells infected with the IBV QX strain.
The IFA results showed that this antibody can effectively recognize the S protein expressed in sf9 cells infected with baculovirus pfast-IBV S and the CEK cells infected with the IBV QX strain.
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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