Anti-H10N3 Avian Influenza HA Protein Mouse Monoclonal Antibody (H10_1F4)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : AXA0009
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Size:
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Product Details
Background
The H10N3 Avian Influenza HA Protein (Hemagglutinin Protein) is a key surface glycoprotein of the H10N3 avian influenza virus, playing an indispensable role in viral entry into host cells and immune recognition. It belongs to class I viral fusion proteins and exists as a homotrimer, with each monomer processed from a precursor (HA0) into two functional subunits (HA1 and HA2) via cleavage by host proteases. The HA1 subunit contains a conserved receptor-binding site (RBS) that specifically binds to α-2,3-linked sialic acid receptors—predominantly expressed on avian respiratory and intestinal epithelial cells. This binding event is the initial step of viral attachment, enabling the virus to anchor to target cells. The HA2 subunit, embedded in the viral envelope, mediates the fusion of the viral membrane with the host cell endosomal membrane through pH-dependent conformational changes, facilitating the release of the viral genome into the cytoplasm for replication.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:200-1:1000 |
IFA | 1:20-1:500 |
Hemagglutination Inhibition | 1:1000-1:2048 |
Overview
Description | Mouse Monoclonal antibody to H10N3 Avian Influenza HA Protein |
Antibody Type | Primary antibody |
Immunogen | H9N2 Avian Influenza Virus |
Form/Buffer | PBS, 20% Glycerol; preservative: 0.05% Sodium Azide |
Alternative Names | H10N3 HA, H10 hemagglutinin, Avian influenza H10 HA, AIV H10N3 hemagglutinin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
The results of WB and IFA indicated that this antibody can effectively recognize the H9N2 HA protein.
The results of WB and IFA indicated that this antibody can effectively recognize the H9N2 HA protein.
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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