Androgen Receptor Rabbit Monoclonal Antibody(ARA748)

Datasheet

MSDS

Androgen Receptor Rabbit Monoclonal Antibody(ARA748)

Key features and details

Target: Androgen Receptor
Source/Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Applications: WB,IHC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6535

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Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues . Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation . Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:5000-1:10000
IHC	1:100-1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to Androgen Receptor
Antibody Type	Primary antibody
Predicted MW	110kDa
Immunogen	A synthetic peptide corresponding to residues on the N-terminus of human AR was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.23%.
Alternative Names	DHTR; NR3C4; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4
Gene Symbol	AR
Entrez Gene	367(Human)
Swissprot	P10275

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-Androgen receptor antibody at 1:5,000 dilution
Predicted MW: 110 kDa
Observed MW: 110 kDa
Lane 1: Rat Testis
Lane 2: Mu Testis

Lysate at 10 µg per lane
2nd Ab:
G&R HRP(H+L) 1:10,000
Exposure: 180s

All lanes: Anti-Androgen receptor antibody at 1:2,000 dilution
Predicted MW: 110 kDa
Observed MW: 110 kDa
Lane 1: T47D
Lane 2: A549

Lysate at 10 µg per lane
2nd Ab:
G&R HRP(H+L) 1:5,000
Exposure: 60s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling Androgen Receptor with ARA748 at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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