Alpha Tubulin Rabbit Polyclonal antibody

Datasheet

MSDS

Alpha Tubulin Rabbit Polyclonal antibody

Key features and details

Target: Alpha Tubulin
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Application: WB, IHC, IP, ICC/IF, FC
Storage: -20°C

Brand:

CAT.NO. : ARA6460

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:2000 - 1:50000
IP	0.4-4.0 µg for 1.0-4.0 mg of total protein lysate
IHC	1:100 - 1:500
IF/ICC	1:100 - 1:500
FC	0.50 µg per 10^6 cells in a 100 µl suspension

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Reactivity	Human, Mouse, Rat
Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant human Tubulin-Alpha protein
Full Name	tubulin, alpha 1b
Calculated Molecular Weight	50 kDa
Observed Molecular Weight	50-55 kDa
Gene Symbol	Alpha Tubulin
Gene ID (NCBI)	10376
Conjugate	Unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Various lysates were subjected to SDS PAGE followed by western blot with Alpha Tubulin Rabbit Polyclonal antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Alpha Tubulin Rabbit Polyclonal antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin Rabbit Polyclonal antibody at dilution of 1:200 and Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Fluor 594-Phalloidin (red).

IP result of Alpha Tubulin Rabbit Polyclonal antibody (IP:3ug; Detection:1:1000) with HeLa cells lysate 5000ug.

1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug Alpha Tubulin Rabbit Polyclonal antibody and Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.5 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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