AKR7L Rabbit Polyclonal Antibody
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:10 - 1:50 |
Description | Rabbit polyclonal antibody to AKR7L |
Specificity | Recognizes endogenous levels of AKR7L protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human AKR7L. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 36 kD; Observed: 36 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | AFAR3; AKR7A4; Aflatoxin B1 aldehyde reductase member 4; AFB1 aldehyde reductase 3; AFB1-AR 3; Aldoketoreductase 7-like |
Gene Symbol | AKR7L |
Entrez Gene | 246181(Human) |
SwissProt | Q8NHP1(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of AKR7L expression in HepG2 (A) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 36 kD)
Immunofluorescent analysis of Anti-AKR7L staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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