AKAP3 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:100 - 1:500 |
Description | Rabbit polyclonal antibody to AKAP3 |
Specificity | Recognizes endogenous levels of AKAP3 protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKAP3. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 94 kD; Observed: 95 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | AKAP110; SOB1; A-kinase anchor protein 3; AKAP-3; A-kinase anchor protein 110 kDa; AKAP 110; Cancer/testis antigen 82; CT82; Fibrous sheath protein of 95 kDa; FSP95; Fibrousheathin I; Fibrousheathin-1; Protein kinase A-anchoring protein 3; PRKA3; Sperm oocyte-binding protein |
Gene Symbol | AKAP3 |
Entrez Gene | 10566(Human); 11642(Mouse) |
SwissProt | O75969(Human); O88987(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of AKAP3 expression in Raw264.7 (A), HeLa (B), H9C2 (C) whole cell lysates. (Predicted band size: 94 kD; Observed band size: 95 kD)
Immunofluorescent analysis of AKAP3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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