AIM2 Mouse Monoclonal Antibody(C2500)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:100 - 1:500 |
Description | Mouse monoclonal to AIM2 |
Specificity | Recognizes endogenous levels of AIM2 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human AIM2 expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 39 kD; Observed: 42 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Interferon-inducible protein AIM2; Absent in melanoma 2 |
Gene Symbol | AIM2 |
Entrez Gene | 9447(Human) |
SwissProt | O14862(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of AIM2 expression in HepG2 (A), A549 (B) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 42 kD kD)
Immunofluorescent analysis of AIM2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
FAQs
New Products
