ACAT2 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:200 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to ACAT2 |
Specificity | Recognizes endogenous levels of ACAT2 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human ACAT2 |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 41; Observed: 41 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | ACTL; Acetyl-CoA acetyltransferase, cytosolic; Acetyl-CoA transferase-like protein; Cytosolic acetoacetyl-CoA thiolase |
Gene Symbol | ACAT2 |
Entrez Gene | 39(Human); 110460(Mouse); 308100(Rat) |
SwissProt | Q9BWD1(Human); Q8CAY6(Mouse); Q5XI22(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of ACAT2 expression in MCF7 (A), HepG2 (B), PC12 (C), mouse liver (D) whole cell lysates. (Predicted band size: 41; 44 kD; Observed band size: 41 kD)
Immunohistochemical analysis of ACAT2 staining in rat ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ACAT2 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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