14-3-3 σ Mouse Monoclonal Antibody (5D7)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : ARA1457
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Product Details
Background
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell cycle checkpoints. Seven iso - forms, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ, comprise this family of signaling intermediates. 14-3-3 σ, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominantly in stratified squamous keratinizing epithelium, 14-3-3 σ is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to KRT17, for example, 14-3-3 σ acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 σ also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 σ positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G₂/M cell cycle progression. Multiple isoforms of 14-3-3 σ exist due to alternative splicing events.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:100-1:1000 |
IHC | 1:50-1:500 |
IF/ICC | 1:50-1:500 |
ELISA | 1:30-1:3000 |
IP | 1-2 µg/100-500 µg total protein |
Overview
Isotype | Mouse IgG1 kappa light chain |
Target Antigen | 14-3-3 sigma |
Gene Name | SFN |
UniProt ID | P31947 (Human), O70456 (Mouse) |
Entrez Gene ID | 2810 (Human), 55948 (Mouse), 313017 (Rat) |
Molecular Weight | 30 kDa |
Gene Aliases | Stratifin, YWHAS, Epithelial cell marker protein 1, HME1 |
Immunogen | Recombinant 14-3-3 sigma of human origin (epitope mapping within amino acids 1-249) |
Form/Buffer | PBS with < 0.1% sodium azide and 0.1% gelatin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of 14-3-3 σ expression in HeLa nuclear extract.
Immunoperoxidase staining of formalin - fixed, paraffin - embedded human uterine cervix tissue showing nuclear and cytoplasmic localization.
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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