VCPIP1 Mouse Monoclonal Antibody(ARA658)

Datasheet

MSDS

VCPIP1 Mouse Monoclonal Antibody(ARA658)

Key features and details

Target: VCPIP1
Host: Mouse
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: WB, IF, IF/ICC, ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA6376

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP. Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:100-1:1000
IP	1-2 µg per 100-500 µg of total protein(1 ml of cell lysate)
IF/ICC	1:50-1:500
ELISA	1:50-1:3000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Isotype	Mouse IgG1 kappa light chain
Target Antigen	VCPIP1 (Valosin-containing protein interacting protein 1)
Gene Name	VCPIP1
UniProt ID	Q96JH7 (Human), Q8CDG3 (Mouse), Q5FVR2/A0A0G2K7V9 (Rat)
Entrez Gene ID	80124 (Human), 70675 (Mouse), 286761 (Rat)
Molecular Weight	135 kDa
Gene Aliases	VCIP135, DUBA3, FLJ23132, KIAA1850
Immunogen	amino acids 84-293 mapping of VCPIP1 of human origin
Formulation	PBS with < 0.1% sodium azide and 0.1% gelatin

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of VCPIP1 expression in MCF7 (A), MDA-MB-231 (B), Hel.92.1.7 (C), NIH/3T3 (D) and SP2/0 (E) whole cell lysates and rat testis tissue extract (F).

Near-Infrared western blot analysis of VCPIP1 expression in K-562 (A) and HEL 92.1.7 (B) whole cell lysates.

Storage

Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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