Tubulin-β Rabbit Monoclonal Antibody(ARA907)

Key features and details

  • Target: Tubulin-β
  • Host: Rabbit
  • Reactivity: Human, Mouse, Rat
  • Clonality: Monoclonal
  • Application: IHC
  • Storage: -20°C
  • Brand:
CAT.NO. : ARA6742
US$ Please choose
US$ Please choose
Size:
1mL
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application

Dilution Ratio

IHC

1:400-1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Overview

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human beta Tubulin aa 308-357 / 444

Molecular Weight

Predicted band size: 50 kDa

Positive Control

Hela cell lysate, 293T cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, U-2 OS cell lysate, Jurkat cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y, HeLa, NIH/3T3, CRC, N2A, PC-12, human fallopian tube tissue, human colon carcinoma tissue, rat kidney tissue, mouse large intestine tissue

Conjugation

unconjugated

Form/Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

Isotype

IgG

Purification

Protein A affinity purified

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data

Immunohistochemical staining of human colon carcinoma tissue using Tubulin-β Rabbit Monoclonal Antibody(ARA907)

Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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