Tpit Mouse Monoclonal Antibody(ARA846)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6670
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Size:
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Product Details
Background
Tpit (TBX19, T box 19) is a highly cell-restricted transcription factor belonging to the T-Box family. T box 19 is required for pro-opeiomelanocortin (POMC) gene expression and differentiation of corticotroph and melanotroph cells. Tpit gene mutations have been shown to be directly correlated to pituitary adrenocorticotropin (ACTH) deficiencies. Tpit functions as a transcriptional activator by recruiting the p160 family of coactivators.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Species Reactivity | Human |
Immunogen | Recombinant fragment corresponding to Human Tpit aa 350 to the C-terminus. Database link: O60806 |
Epitope | Binds to an epitope located within the peptide sequence VLGEPSLTSIAVSTW as determined by overlapping synthetic peptides |
Form | Liquid |
Storage Solution | pH: 7.20; Preservative: 0.02% Sodium azide; Constituents: 40% Glycerol (glycerin, glycerine), PBS |
Purity | Protein A purified |
Purification Description | Purified from TCS |
Clone | Monoclonal |
Isotype | IgG1 |
Entrez Gene | 9095 (Human) |
Omim | 604614 (Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Formalin-fixed, paraffin-embedded human pituitary gland tissue stained for Tpit in immunohistochemical analysis.
Negative control - Formalin-fixed, paraffin-embedded human tonsil tissue stained for Tpit at in immunohistochemical analysis.
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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