RAD52 Mouse Monoclonal Antibody(C2932)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:100 - 1:500 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to RAD52 |
Specificity | Recognizes endogenous levels of RAD52 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human RAD52 expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 46 kD; Observed: 50 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | DNA repair protein RAD52 homolog |
Gene Symbol | RAD52 |
Entrez Gene | 5893(Human) |
SwissProt | P43351(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of RAD52 expression in HepG2 (A), MCF7 (B), MCF7 (C), C6 (D) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 50 kD kD)
Immunofluorescent analysis of RAD52 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of MCF7 cells using Anti-RAD52 Antibody (green) and negative control (red).
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