RAB11A Rabbit Polyclonal Antibody
Key features and details
- Reactivity:
- Application:
- Host:
- Clonality:
- Target:
-
Brand:
CAT.NO. : ARA6368
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Size:
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Product Details
Background
Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500-1:1000 |
|
IHC |
1:50-1:200 |
|
IP |
1:200-1:800 |
|
IF/ICC |
0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Overview
|
Recommended Method |
WB, IHC, IF/ICC, IP, ELISA |
|
Reactivity Species |
Human, Mouse, Rat |
|
Immunogen |
Peptide |
|
Host Species / Isotype |
Rabbit / IgG |
|
Clonality |
Polyclonal |
|
Product Type |
Antibody |
|
Alternative Names |
RAB11A-Specific, Rab 11, RAB11, RAB11A/B, EC:3.6.5.2 |
|
Calculated Molecular Weight |
24 kDa |
|
Observed Molecular Weight |
24 kDa |
|
Gene Symbol |
RAB11A |
|
Gene ID (Human) |
8766 |
|
Conjugate |
Unconjugated |
|
Form |
Liquid |
|
Purification Method |
Antigen affinity purification |
|
Protein ID (Human) |
P62491 |
|
Storage Buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Data
Rat brain tissue were subjected to SDS PAGE followed by western blot with RAB11A Rabbit Polyclonal Antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using RAB11A Rabbit Polyclonal Antibody at dilution of 1:200.
IP result of RAB11A Rabbit Polyclonal Antibody, 4ug; Detection:RAB11A Rabbit Polyclonal Antibody 1:1000) with SH-SY5Y cells lysate 1840 ug.
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RAB11A Rabbit Polyclonal Antibody at dilution of 1:400 and AREX®Flour 488-Conjugated Rabbit IgG(H+L) Goat Antibody, AREX®Flour 594-Phalloidin (red).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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