RAB11A Rabbit Polyclonal Antibody

Datasheet

MSDS

Key features and details

Target: RAB11A
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Application: WB, IHC, IF/ICC, IP, ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA6368

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:500-1:1000
IHC	1:50-1:200
IP	1:200-1:800
IF/ICC	0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Recommended Method	WB, IHC, IF/ICC, IP, ELISA
Reactivity Species	Human, Mouse, Rat
Immunogen	Peptide
Host Species / Isotype	Rabbit / IgG
Clonality	Polyclonal
Product Type	Antibody
Alternative Names	RAB11A-Specific, Rab 11, RAB11, RAB11A/B, EC:3.6.5.2
Calculated Molecular Weight	24 kDa
Observed Molecular Weight	24 kDa
Gene Symbol	RAB11A
Gene ID (Human)	8766
Conjugate	Unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Rat brain tissue were subjected to SDS PAGE followed by western blot with RAB11A Rabbit Polyclonal Antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using RAB11A Rabbit Polyclonal Antibody at dilution of 1:200.

IP result of RAB11A Rabbit Polyclonal Antibody, 4ug; Detection:RAB11A Rabbit Polyclonal Antibody 1:1000) with SH-SY5Y cells lysate 1840 ug.

Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RAB11A Rabbit Polyclonal Antibody at dilution of 1:400 and AREX®Flour 488-Conjugated Rabbit IgG(H+L) Goat Antibody, AREX®Flour 594-Phalloidin (red).

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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