Prohibitin Rabbit Monoclonal Antibody(ARA729)

Datasheet

MSDS

Prohibitin Rabbit Monoclonal Antibody(ARA729)

Key features and details

Target: Prohibitin
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: WB, IHC, IF/ICC, IP
Storage: -20°C

Brand:

CAT.NO. : ARA6516

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:500 - 1:1000
IF/ICC	1:100 - 1:1000
IHC	1:200 - 1:1000
IP	1-2μg/sample
FC	1:800 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide within Human Prohibitin aa 147-196 / 272.
Molecular Weight	Predicted band size: 30 kDa
Positive Control	HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate, hybrid fish (crucian-carp) liver tissue lysates, HepG2, PC-12, NIH/3T3, human liver carcinoma tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue, mouse stomach tissue, mouse heart tissue, human liver tissue.
Conjugation	unconjugated
Form/Buffer	1×TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype	IgG
Purification	Protein A affinity purified.

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Prohibitin on different lysates with Prohibitin Rabbit Monoclonal Antibody(ARA729).

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Prohibitin Rabbit Monoclonal Antibody(ARA729).

Prohibitin was immunoprecipitated from 0.2 mg HEK-293 cell lysate with Prohibitin Rabbit Monoclonal Antibody(ARA729).

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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