PE-labled Human IgG1 Mouse Monoclonal Antibody(ARA651)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Conjugation:
- Storage:
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Brand:
CAT.NO. : ARA6361
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Product Details
Background
Human Immunoglobulin G subclass 1 (IgG1) is the most abundant antibody subclass in human serum, constituting approximately 60-70% of total IgG. It serves as a critical mediator of the adaptive immune system's defense against pathogens. Human IgG1 represents a perfect balance of specificity, stability, and powerful immune-activating capacity. Its dominance in the biotherapeutic landscape is a testament to its versatility and effectiveness. This antibody does not recognize IgA2.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:1000 |
IF/ICC | 1:100 - 1:1000 |
ELISA | At an assay dependent concentration |
FC | 1:1000 - 1:4000 |
Overview
Product Description | Mouse Monoclonal Secondary Antibody to Human IgG1 PE labeled |
Immunogen | Human IgG1 |
Purification | The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Clonality | Monoclonal |
Conjugate | PE |
Form | 1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide |
Detection Platform | Ex/Em = 565/576 nm |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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