PAI-2 Mouse Monoclonal Antibody (E-1)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : ARA0201
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Product Details
Background
PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the Serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase - type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the Serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPa is a serine proteinase that is a member of the Trypsin family. It is responsible for the cleavage of plasminogen at the Arg - Val bond to produce plasmin. uPA consists of two chains, designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:100-1:1000 |
IF/ICC | 1:50-1:500 |
ELISA | 1:30-1:3000 |
IP | 1-2 µg/100-500 µg total protein |
Overview
Isotype | Mouse IgG1 kappa light chain |
Target Antigen | PAI-2 (Plasminogen activator inhibitor 2) |
Gene Name | SERPINB2 |
UniProt ID | P05120 (Human), P12388 (Mouse) |
Entrez Gene ID | 5055 (Human), 18788 (Mouse) |
Molecular Weight | 47 kDa (cytoplasmic form), 60 kDa (glycosylated extracellular form) |
Gene Aliases | PAI2, PLANH2, Serpin B2, Monocyte-derived plasminogen activator inhibitor |
Immunogen | amino acids 116-245 mapping within an internal region of PAI-2 of human origin |
Form/Buffer | PBS with < 0.1% sodium azide and 0.1% gelatin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of PAI-2 expression in K-562 (A), WI-38 (B), C2C12 (C), WEHI-231 (D) and A-10 (E) whole cell lysates.
Western blot analysis of PAI-2 expression in K-562 (A), JAR (B) and A-431 (C) whole cell lysates.
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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