LRP1 Rabbit Monoclonal Antibody(ARA839)

Datasheet

MSDS

Key features and details

Target: LRP1
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: IHC, WB, IP
Storage: -20°C

Brand:

CAT.NO. : ARA6654

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

LRP1 is a member of the LDLR family and ubiquitously expressed in multiple tissues, though it is most abundant in vascular smooth muscle cells (SMCs), hepatocytes, and neurons. LRP1 plays a key role in intracellular signaling and endocytosis, which thus implicate it in many cellular and biological processes, including lipid and lipoprotein metabolism, protease degradation, platelet derived growth factor receptor regulation, integrin maturation and recycling, regulation of vascular tone, regulation of blood brain barrier permeability, cell growth, cell migration, inflammation, and apoptosis, as well as diseases such as neurodegenerative diseases, atherosclerosis, and cancer. The LRP1 gene encodes a 600 kDa precursor protein that is processed by furin in the trans-Golgi complex, resulting in a 515 kDa alpha-chain and an 85 kDa beta-chain associated noncovalently.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio / Usage
WB	1:1,000-1:5,000
IF/ICC	1-25:1:50
IHC	1:200-1:2,000
IP	1-4µg/sample

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Product Name	LRP1 Recombinant Rabbit Monoclonal Antibody
Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide within Human LRP1 aa 4417-4502 /4544.
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IF-Tissue, IHC-P, IP, IHC-Fr
Molecular Weight	Predicted band size: 505 kDa
Positive Control	Mouse liver tissue lysate, Human lung tissue lysate, Rat liver tissue lysate, Rat lung tissue lysate, Mouse lung tissue lysate, Human liver tissue lysate, mouse brain tissue, rat brain tissue, rat liver tissue, human lung tissue, human liver tissue, mouse liver tissue.
Conjugation	unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LRP1 antibody (ARA839) at 1/2,000 dilution.

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-LRP1 antibody (ARA839) at 1/2,000 dilution.

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-LRP1 antibody (ARA839) at 1/5,000 dilution.

Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-LRP1 antibody (ARA839) at 1/200 dilution.

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LRP1 antibody (ARA839) at 1/2,000 dilution.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-LRP1 antibody (ARA839) at 1/200 dilution.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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