IL1RAPL1 Mouse Monoclonal Antibody(C2769)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:100 - 1:500 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to IL1RAPL1 |
Specificity | Recognizes endogenous levels of IL1RAPL1 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human IL1RAPL1 expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 80 kD; Observed: 83 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | OPHN4; Interleukin-1 receptor accessory protein-like 1; IL-1-RAPL-1; IL-1RAPL-1; IL1RAPL-1; Oligophrenin-4; Three immunoglobulin domain-containing IL-1 receptor-related 2; TIGIRR-2; X-linked interleukin-1 receptor accessory protein-like 1 |
Gene Symbol | IL1RAPL1 |
Entrez Gene | 11141(Human) |
SwissProt | Q9NZN1(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of IL1RAPL1 expression in A431 (A), SKHep1 (B), HL7702 (C) whole cell lysates. (Predicted band size: 80 kD; Observed band size: 83 kD kD)
Immunofluorescent analysis of IL1RAPL1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HeLa cells using Anti-IL1RAPL1 Antibody (green) and negative control (red).
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