Human Nectin-4 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0151
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0126 |
0.0126 |
0.0126 |
|
|
2.74 |
0.0216 |
0.0205 |
0.0211 |
0.0085 |
|
8.23 |
0.0338 |
0.0367 |
0.0353 |
0.0227 |
|
24.69 |
0.0781 |
0.0753 |
0.0767 |
0.0641 |
|
74.07 |
0.2027 |
0.2158 |
0.2093 |
0.1967 |
|
222.22 |
0.6049 |
0.6287 |
0.6168 |
0.6042 |
|
666.67 |
1.7850 |
1.8030 |
1.7940 |
1.7814 |
|
2000.00 |
3.6480 |
3.6120 |
3.6300 |
3.6174 |
Precision
|
|
Intra-assay Precision |
Inter-assay Precision |
||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
49.8 |
240.7 |
861.5 |
44.4 |
211.8 |
614.5 |
|
Standard Deviation |
1.9 |
11.5 |
27.1 |
1.3 |
12.3 |
35.4 |
|
Coefficient of Variation(%) |
3.8 |
4.8 |
3.1 |
3.0 |
5.8 |
5.8 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Nectin-4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 100% to 117% with an overall mean recovery of 109%.
The recovery ranged from 100% to 117% with an overall mean recovery of 109%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 56.23-451.61 | 100 | 226.49 |
New Products
