Human CD63 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0167
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Size:
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Product Details
Background
CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0151 |
0.0128 |
0.0140 |
|
|
6.86 |
0.0286 |
0.0280 |
0.0283 |
0.0144 |
|
20.58 |
0.0572 |
0.0582 |
0.0577 |
0.0438 |
|
61.73 |
0.1475 |
0.1458 |
0.1467 |
0.1327 |
|
185.19 |
0.4194 |
0.3984 |
0.4089 |
0.3950 |
|
555.56 |
1.2120 |
1.1120 |
1.1620 |
1.1481 |
|
1666.67 |
2.6950 |
2.5740 |
2.6345 |
2.6206 |
|
5000.00 |
3.8760 |
3.8910 |
3.8835 |
3.8696 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
336.6 |
1807.6 |
5114.7 |
325.3 |
1844.1 |
5419.5 |
|
Standard Deviation |
10.2 |
76.5 |
268.5 |
11.8 |
98.5 |
124.0 |
|
Coefficient of Variation(%) |
3.0 |
4.2 |
5.2 |
3.6 |
5.3 |
2.3 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD63 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 119% with an overall mean recovery of 105%.
The recovery ranged from 93% to 119% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 570.74-1262.25 | 100 | 787.53 |
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