Human beta Amyloid 1-40 ELISA Kit
Protocol Booklet 
Key features and details
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Brand:
CAT.NO. : AEH0111
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Product Details
Background
β-Amyloid (1-40), Iowa mutation is a biologically active peptide. (Several mutations in the beta amyloid precursor gene lead to autosomal dominant Alzheimer's disease in many families. Iowa mutations (where Asp 23 is replaced by Asn) are associated with severe brain amyloid vascular disease (CAA). The affected individuals have a missense mutation at APP 694. The mutated beta-amyloid peptides accumulate faster and form toxic fibrils
typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0446 |
0.0451 |
0.0449 |
|
|
31.25 |
0.0817 |
0.0770 |
0.0794 |
0.0345 |
|
62.50 |
0.1291 |
0.1349 |
0.1320 |
0.0871 |
|
125.00 |
0.2772 |
0.2812 |
0.2792 |
0.2343 |
|
250.00 |
0.5233 |
0.5129 |
0.5181 |
0.4732 |
|
500.00 |
1.0246 |
1.0388 |
1.0317 |
0.9868 |
|
1000.00 |
1.8213 |
1.8056 |
1.8135 |
1.7686 |
|
2000.00 |
3.3130 |
3.3240 |
3.3185 |
3.2736 |
precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average（pg/ml） |
757.9 |
168.5 |
48.0 |
703.8 |
202.7 |
42.7 |
|
Standard deviation |
40.9 |
7.1 |
1.8 |
22.1 |
5.6 |
2.4 |
|
Coefficient of variation（%） |
5.4 |
4.2 |
3.7 |
3.1 |
2.8 |
5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision
spike recovery
The spike recovery was evaluated by spiking 3 levels of human beta Amyloid 1-40 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 122% with an overall mean recovery of 105%.
The recovery ranged from 84% to 122% with an overall mean recovery of 105%.
sample values
|
Sample Matrix |
Sample Evaluated |
Range (pg/ml) |
Detectable % |
Mean of Detectable (pg/ml) |
|
Serum |
30 |
n.d.- 907.22 |
37.50 |
198.85 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of beta Amyloid 1-40 in this assay. No medical histories were available for the donors.
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