H+/K+ ATPase β Mouse Monoclonal Antibody(ARA827)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6328
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Size:
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Product Details
Background
The gastric H⁺/K⁺ ATPase exists as a heterodimer consisting of an α and a β subunit that work in tandem to transport protons across plasma membranes. H⁺/K⁺ ATPase β, also known as ATP4B or ATP6B, is a 291 amino acid single - pass type II membrane protein that functions as the β subunit of the H⁺/K⁺ ATPase heterodimer. Working with the α subunit, H⁺/K⁺ ATPase β effectively catalyzes the hydrolysis of ATP coupled with the exchange of H⁺ and K⁺ ions across the plasma membrane and plays an essential role in gastric acid secretion. The gene encoding H⁺/K⁺ ATPase β maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
|
Application |
Dilution Ratio |
|
IHC |
1:50 - 1:500 |
Overview
Isotype | IgG1 kappa light chain |
Research Area | Metabolism, Channels & Transporters, Circulatory System, Hydrogen Potassium ATPase |
Target Antigen | H+/K+ ATPase beta |
Molecular Weight | 35-50 kDa (unglycosylated), 60-80 kDa (glycosylated) |
Gene Aliases | ATP4B, Gastric Hydrogen Potassium ATPase Beta Subunit |
Immunogen | amino acids 1-291 representing full length $H^+/K^+$ ATPase $\beta$ of human origin |
Form/Buffer | PBS with < 0.1% sodium azide and 0.1% gelatin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing membrane staining of glandular cells.
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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