EGFR Mouse Monoclonal Antibody(C3486)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
IP | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to EGFR |
Specificity | Recognizes endogenous levels of EGFR protein. |
Antibody Type | Primary antibody |
Imnunogen | Purified recombinant human EGFR protein fragments expressed in E.coli. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 134 kD; Observed: 175 kD |
Form/Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
Alternative Names | ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 |
Gene Symbol | EGFR |
Entrez Gene | 1956(Human) |
SwissProt | P00533(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of EGFR expression in A549 (A), MDAMB468 (B), COS7 (C) whole cell lysates. (Predicted band size: 134 kD; Observed band size: 175 kD)
Immunofluorescent analysis of EGFR staining in MDAMB468 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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