CHD4 Mouse Monoclonal Antibody(C3357)

MSDS

Key features and details

Mouse monoclonal antibody to CHD4

Target: CHD4
Source/Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Applications: WB, IP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA02969

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA . Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin . Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination . Involved in neurogenesis .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IP	1:10 - 1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Mouse monoclonal antibody to CHD4
Specificity	Recognizes endogenous levels of CHD4 protein.
Antibody Type	Primary antibody
Imnunogen	Purified recombinant human CHD4 protein fragments expressed in E.coli.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 218 kD; Observed: 260 kD
Form/Buffer	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Alternative Names	Chromodomain-helicase-DNA-binding protein 4; CHD-4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta
Gene Symbol	CHD4
Entrez Gene	1108(Human)
SwissProt	Q14839(Human)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of CHD4 expression in K562 (A), Hela (B), HL60 (C), Molt4 (D), Jurkat (E), 293T (F) whole cell lysates. (Predicted band size: 218 kD; Observed band size: 260 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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