CD3e Rabbit Polyclonal Antibody
Datasheet 
Key features and details
Rabbit polyclonal antibody to CD3e
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
CAT.NO. : APA10249
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Product Details
Background
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response . When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z . All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain . Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways . CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
Overview
Description | Rabbit polyclonal antibody to CD3e |
Specificity | Recognizes endogenous levels of CD3e protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD3e. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 23 kD; Observed: 21 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e |
Gene Symbol | CD3E |
Entrez Gene | 916(Human) |
SwissProt | P07766(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of CD3e expression in Jurkat (A), Hela (B), NIH3T3 (C) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 21 kD)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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