CD34 Rabbit Monoclonal Antibody(ARA815)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:2000-1:5000 |
IHC | 1:6400-1:12800 |
IF/ICC | 1:200-1:800 |
FC | 1:2000-1:10000 |
IP | 1:100 |
Description | Rabbit Monoclonal Antibody to CD34 |
Antibody Type | Primary antibody |
Predicted MW | 41kDa |
Immunogen | A synthetic peptide corresponding to residues on the C-terminus of human CD34 was used as an immunogen. |
Purification | ProA affinity purified IgG |
Form/Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.90%. |
Alternative Names | Hematopoietic progenitor cell antigen CD34; CD34 |
Gene Symbol | CD34 |
Entrez Gene | 947(Human) |
Swissprot | P28906 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
All lanes: Anti-CD34 antibody at 1:5,000 dilution
Predicted MW: 41 kDa
Observed MW: 120 kDa
Lane 1: TF-1
Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 60s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD34 with ARA815 at 1:12,800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
ARA815 staining CD34 in TF-1 cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:200) at 4°C. An Alexa Fluor<sup>®</sup> 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor<sup>®</sup> 488-conjugated Goat Anti-Rabbit IgG (1:500).
Overlay histogram showing TF-1 cells stained with ARA815 (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA815, 1:10,000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor<sup>®</sup> 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
CD34 was immunoprecipitated from 0.4mg of TF-1 whole cell lysate with ARA815 at 1:100 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: ARA815 IP in TF-1 whole cell lysate
Lane 2: PBS instead of ARA815 in TF-1 whole cell lysate
Lane 3: TF-1 whole cell lysate, 10 µg (input)
Exposure: 120s
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