CCDC122 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:200 |
FC | 1:10 - 1:50 |
Description | Rabbit polyclonal antibody to CCDC122 |
Specificity | Recognizes endogenous levels of CCDC122 protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human CCDC122. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 32 kD; Observed: 40 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Coiled-coil domain-containing protein 122 |
Gene Symbol | CCDC122 |
Entrez Gene | 160857(Human) |
SwissProt | Q5T0U0(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of CCDC122 expression in CCRFCEM (A), 293 (B) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 40 kD)
Immunohistochemical analysis of CCDC122 staining in human bladder carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of CEM cells using Anti-CCDC122 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
FAQs
New Products
