Biotin-conjugated Rabbit IgG (H&L) Goat Polyclonal Antibody
Key features and details
- Reactivity:Rabbit
- Host:Goat
- Conjugation:Biotin
- Application:WB, IHC, ELISA, IF/ICC
- Target:Rabbit IgG
-
Brand:
CAT.NO. : APA19559
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US$ Please choose
Product Details
Background
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
Application
|
Application |
Dilution Ratio |
|
WB |
1:5000 - 1:20000 |
|
IHC |
1:500 - 1:2000 |
|
ELISA |
1:5000 - 1:20000 |
|
IF/ICC |
1:500 - 1:2000 |
Overview
|
Product Description |
Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) Biotin |
|
Immunogen |
Rabbit IgG |
|
Purification Method |
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
|
Clonality |
Polyclonal |
|
Conjugate |
Biotin |
|
Form |
1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.05% Sodium Azide |
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
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