Anti-Canine Coronavirus (CCoV) N Protein Mouse Monoclonal Antibody (13G8)
Key features and details
- Clone:
- Host:
- Species Reactivity:
- Application:
- Immunogen :
- Clone Type:
- Storage:
-
Brand:
CAT.NO. : AXA0035
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Size:
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Product Details
Background
Canine Coronavirus (CCoV) N Protein is a core nucleocapsid protein of CCoV, responsible for packaging the viral genomic RNA into ribonucleocapsids. It plays key roles in viral replication, assembly, and subgenomic RNA transcription. With high immunogenicity, it serves as an important target for the development of CCoV diagnostic reagents and subunit vaccines.
Overview
|
Host |
Mouse |
|
Clonality |
Monoclonal |
|
Use |
WB, IF, ELISA |
|
Reactivity |
CCoV N Protein |
|
Antibody Isotype |
IgG1 |
|
Appearance |
Liquid |
|
Storage Buffer |
PBS, 20% Glycerol; preservative: 0.05% Sodium Azide |
|
Storage Condition |
Store at -20°C, valid for 1 year, avoid repeated freeze-thaw cycles. |
|
Concentration |
1 mg/mL |
|
Immunogen |
CCoV Whole Virus |
Application
|
Application |
Recommended Dilution Ratio |
|
WB |
1:200-1:1000 |
|
IF |
1:200-1:1000 |
|
ELISA |
1:5000-1:10000 |
*The optimal dilution ratio/concentration should be determined by researchers.
Data
The WB results showed that this antibody can effectively recognize the CCoV N protein. The IFA results indicated that this antibody can effectively recognize CCoV-infected cells.
Reference
1. 甘军纪，吕海峰，汤也，田晓彦，刘秀梵. 犬冠状病毒M和N蛋白单克隆抗体的制备及其生物学特性鉴定,中国兽医学报,2021, 41(8),1482-1489.
2. Xiaoyan Tian, Ye Tang, Junji Gan, Jianqiang Ye. A novel linear B cell epitope of the canine coronavirus nucleocapsid protein identified by a monoclonal antibody. Veterinary Microbiology 293 (2024) 110098.
2. Xiaoyan Tian, Ye Tang, Junji Gan, Jianqiang Ye. A novel linear B cell epitope of the canine coronavirus nucleocapsid protein identified by a monoclonal antibody. Veterinary Microbiology 293 (2024) 110098.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Intended use
For Research Use Only. Not for use in diagnostic procedures.
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