ZFP91 Mouse Monoclonal Antibody(C3052)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:100 - 1:500 |
IF/ICC | 1:50 - 1:250 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to ZFP91 |
Specificity | Recognizes endogenous levels of ZFP91 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human ZFP91 expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 63 kD; Observed: 63, 105 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | ZNF757; E3 ubiquitin-protein ligase ZFP91; Zinc finger protein 757; Zinc finger protein 91 homolog; Zfp-91 |
Gene Symbol | ZFP91 |
Entrez Gene | 80829(Human) |
SwissProt | Q96JP5(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of ZFP91 expression in Jurkat (A), A431 (B), HepG2 (C), HEK293 (D), A549 (E), PC3 (F) whole cell lysates. (Predicted band size: 63 kD; Observed band size: 63, 105 kD kD)
Immunohistochemical analysis of ZFP91 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ZFP91 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HeLa cells using Anti-ZFP91 Antibody (green) and negative control (red).
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