Vinculin Rabbit Monoclonal Antibody(C3208)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
IP | 1:10 - 1:50 |
Description | Rabbit monoclonal antibody to Vinculin |
Specificity | Recognizes endogenous levels of Vinculin protein. |
Antibody Type | Primary antibody |
Imnunogen | A synthetic peptide of human Vinculin |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 124 kD; Observed: 124 kD |
Form/Buffer | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. |
Alternative Names | Vinculin; Metavinculin; MV |
Gene Symbol | VCL |
Entrez Gene | 7414(Human); 22330(Mouse); 305679(Rat) |
SwissProt | P18206(Human); Q64727(Mouse); P85972(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Vinculin expression in K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates. (Predicted band size: 124 kD; Observed band size: 124 kD)
Immunofluorescent analysis of Vinculin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
FAQs
New Products
