Vimentin Rabbit Monoclonal Antibody(ARB570)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARB6862
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Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is essential in the role of cell integrity and cytoskeletal stability. The reorganization of vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site - specific phosphorylation (serine and threonine residues).Vimentin is expressed in a wide variety of mesenchymal cell types: fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. However, in non - vascular smooth muscle cells, vimentin is often replaced by desmin. In striated muscle, vimentin is also replaced by desmin. However, during regeneration, vimentin is reexpressed. Cells of the lympho - hematopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. In tumor tissues, it is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells.In combination with a panel of antibodies, it is used to identify tumor with mesenchymal origin and malignant melanoma. Additionally, vimentin is a useful control marker for proper tissue processing.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Predicted Molecular Wt | 54kDa |
Purity | ProA affinity purified IgG |
Subcellular location | Cytoplasm |
Swissprot ID | P08670 |
Immunogen | Synthetic peptide corresponding to Vimentin residues within aa366 - 466 of Vimentin was used as an immunogen |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Recommended method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human breast cancer tissue using Vimentin Rabbit Monoclonal Antibody(ARB570)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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