Primary Antibody Diluent
Key features and details
- Product Name: Primary Antibody Diluent
- pH: 7.2-7.4
- Store at 2 - 8°C
-
Brand:
CAT.NO. : ARB0004
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Size:
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Product Details
Intended Use
This product is used for diluting and liquefying test samples to facilitate detection of the target analyte using in vitro diagnostic reagents (after registration) or instruments. It does not directly participate in the detection process itself.
Detection Principle
The sample dilution buffer contains antibody protectants that help maintain antibody activity and stability, reduce non-specific binding, lower non-specific background staining, and improve the specificity and sensitivity of the experiment.
The primary antibody should be diluted to the optimal concentration with this sample dilution buffer according to the conditions optimized by the user through experimentation.
The primary antibody should be diluted to the optimal concentration with this sample dilution buffer according to the conditions optimized by the user through experimentation.
Main Components
This product consists of PBS buffer, protein protectant, and ProClin™ 950.
Storage Conditions and Shelf Life
Storage Conditions: 2–8°C
Shelf Life: 18 months
Please use within 3 months after opening.
Production Date / Expiration Date: See label.
Shelf Life: 18 months
Please use within 3 months after opening.
Production Date / Expiration Date: See label.
Sample Requirements
Suitable for diluting primary antibodies in various types of immunohistochemistry (IHC), ELISA, and other immunological experiments. It is not recommended for the dilution of HRP-labeled antibodies.
Detection Method
1.Preparation: Under sterile conditions, take an appropriate amount of sample dilution buffer according to the manufacturer's instructions or user settings.
2.Mix the diluted antibody working solution thoroughly and set aside for use.
3.Improper storage of the sample dilution buffer can easily lead to protein degradation and loss of activity.Avoid repeated freeze-thaw cycles.
2.Mix the diluted antibody working solution thoroughly and set aside for use.
3.Improper storage of the sample dilution buffer can easily lead to protein degradation and loss of activity.Avoid repeated freeze-thaw cycles.
Interpretation of Test Results
The interpretation of results for immunological experiments such as immunohistochemistry and ELISA should be performed by qualified physicians.
Limitations of the Detection Method
1.Immunohistochemistry, ELISA, and other immunological experiments are multi-step processes. Non-standard operations during the dilution process may affect the final experimental results.
2.Professional operators and certified laboratories will help achieve standardization of the testing process, thereby reducing operational deviations caused by various external factors.
2.Professional operators and certified laboratories will help achieve standardization of the testing process, thereby reducing operational deviations caused by various external factors.
Product Performance Indicators
pH: 7.2 – 7.4
Precautions
1.This reagent is intended for research or raw material development use only and should not be used for any other purposes.
2.Read this instruction manual carefully before starting the experiment.
3.Please use the reagent within its expiration date.
4.This reagent is intended for use by personnel with professional experience or those who have received professional training.
5.Avoid contact of the reagent with eyes and mucous membranes. If contact occurs with sensitive areas, rinse immediately with plenty of water.
6.Adopt appropriate protective measures, such as wearing latex gloves, masks, goggles, lab coats, etc., for safe operation.
7.All waste generated during use should be disposed of in accordance with the “Medical Waste Management Regulations”.
2.Read this instruction manual carefully before starting the experiment.
3.Please use the reagent within its expiration date.
4.This reagent is intended for use by personnel with professional experience or those who have received professional training.
5.Avoid contact of the reagent with eyes and mucous membranes. If contact occurs with sensitive areas, rinse immediately with plenty of water.
6.Adopt appropriate protective measures, such as wearing latex gloves, masks, goggles, lab coats, etc., for safe operation.
7.All waste generated during use should be disposed of in accordance with the “Medical Waste Management Regulations”.
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